| 质粒名称: | pY001 (pFnCpf1_full) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 69973 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pACYC184 |
| 载体出品公司: | New England Biolabs |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 4245 bp |
| 完整质粒大小: | 10715 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | FnCpf1 locus,Cpf1 |
| 物种来源: | 土拉弗朗西斯亚种。诺维西达 |
| 插入大小: | 6569 bp |
| 基因突变: | -- |
| GenBank编号 | WP_003040289.1 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pY001 (pFnCpf1_full) was a gift from Feng Zhang (Addgene plasmid # 69973 ; http://n2t.net/addgene:69973 ; RRID:Addgene_69973) |
| 参考文献部分: | Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. 10.1016/j.cell.2015.09.038 PubMed 26422227 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pY001 (pFnCpf1_full) 10653 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR
array..
ACCESSION .
VERSION .
KEYWORDS pY001 (pFnCpf1_full)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10653)
AUTHORS Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS,
Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin
EV, Zhang F
TITLE Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas
System.
JOURNAL Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi:
10.1016/j.cell.2015.09.038.
PUBMED 26422227
REFERENCE 2 (bases 1 to 10653)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10653
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 134..153
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(220..322)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(714..731)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(848..1393)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(928..947)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS 7989..9179
/codon_start=1
/gene="tet"
/product="tetracycline efflux protein"
/label=TcR
/note="confers resistance to tetracycline"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
primer_bind complement(8341..8360)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS complement(10213..219)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tattttgatg caatatttgt
1441 cttaggtcat ccaagttatt atccaaaatt tggttttaaa ccagccacag aatatcagat
1501 aaaatgtgaa tatgatgtcc cagcggatgt ttttatggta ctagatttgt cagctaaact
1561 agctagttta aaaggacaaa ctgtctacta tgccgatgag tttggcaaaa ttttttagat
1621 ctacaaaatt ataaactaaa taaagattct tataataact ttatatataa tcgaaatgta
1681 gagaatttta taaggagtct ttatcatgtc aatttatcaa gaatttgtta ataaatatag
1741 tttaagtaaa actctaagat ttgagttaat cccacagggt aaaacacttg aaaacataaa
1801 agcaagaggt ttgattttag atgatgagaa aagagctaaa gactacaaaa aggctaaaca
1861 aataattgat aaatatcatc agttttttat agaggagata ttaagttcgg tttgtattag
1921 cgaagattta ttacaaaact attctgatgt ttattttaaa cttaaaaaga gtgatgatga
1981 taatctacaa aaagatttta aaagtgcaaa agatacgata aagaaacaaa tatctgaata
2041 tataaaggac tcagagaaat ttaagaattt gtttaatcaa aaccttatcg atgctaaaaa
2101 agggcaagag tcagatttaa ttctatggct aaagcaatct aaggataatg gtatagaact
2161 atttaaagcc aatagtgata tcacagatat agatgaggcg ttagaaataa tcaaatcttt
2221 taaaggttgg acaacttatt ttaagggttt tcatgaaaat agaaaaaatg tttatagtag
2281 caatgatatt cctacatcta ttatttatag gatagtagat gataatttgc ctaaatttct
2341 agaaaataaa gctaagtatg agagtttaaa agacaaagct ccagaagcta taaactatga
2401 acaaattaaa aaagatttgg cagaagagct aacctttgat attgactaca aaacatctga
2461 agttaatcaa agagtttttt cacttgatga agtttttgag atagcaaact ttaataatta
2521 tctaaatcaa agtggtatta ctaaatttaa tactattatt ggtggtaaat ttgtaaatgg
2581 tgaaaataca aagagaaaag gtataaatga atatataaat ctatactcac agcaaataaa
2641 tgataaaaca ctcaaaaaat ataaaatgag tgttttattt aagcaaattt taagtgatac
2701 agaatctaaa tcttttgtaa ttgataagtt agaagatgat agtgatgtag ttacaacgat
2761 gcaaagtttt tatgagcaaa tagcagcttt taaaacagta gaagaaaaat ctattaaaga
2821 aacactatct ttattatttg atgatttaaa agctcaaaaa cttgatttga gtaaaattta
2881 ttttaaaaat gataaatctc ttactgatct atcacaacaa gtttttgatg attatagtgt
2941 tattggtaca gcggtactag aatatataac tcaacaaata gcacctaaaa atcttgataa
3001 ccctagtaag aaagagcaag aattaatagc caaaaaaact gaaaaagcaa aatacttatc
3061 tctagaaact ataaagcttg ccttagaaga atttaataag catagagata tagataaaca
3121 gtgtaggttt gaagaaatac ttgcaaactt tgcggctatt ccgatgatat ttgatgaaat
3181 agctcaaaac aaagacaatt tggcacagat atctatcaaa tatcaaaatc aaggtaaaaa
3241 agacctactt caagctagtg cggaagatga tgttaaagct atcaaggatc ttttagatca
3301 aactaataat ctcttacata aactaaaaat atttcatatt agtcagtcag aagataaggc
3361 aaatatttta gacaaggatg agcattttta tctagtattt gaggagtgct actttgagct
3421 agcgaatata gtgcctcttt ataacaaaat tagaaactat ataactcaaa agccatatag
3481 tgatgagaaa tttaagctca attttgagaa ctcgactttg gctaatggtt gggataaaaa
3541 taaagagcct gacaatacgg caattttatt tatcaaagat gataaatatt atctgggtgt
3601 gatgaataag aaaaataaca aaatatttga tgataaagct atcaaagaaa ataaaggcga
3661 gggttataaa aaaattgttt ataaactttt acctggcgca aataaaatgt tacctaaggt
3721 tttcttttct gctaaatcta taaaatttta taatcctagt gaagatatac ttagaataag
3781 aaatcattcc acacatacaa aaaatggtag tcctcaaaaa ggatatgaaa aatttgagtt
3841 taatattgaa gattgccgaa aatttataga tttttataaa cagtctataa gtaagcatcc
3901 ggagtggaaa gattttggat ttagattttc tgatactcaa agatataatt ctatagatga
3961 attttataga gaagttgaaa atcaaggcta caaactaact tttgaaaata tatcagagag
4021 ctatattgat agcgtagtta atcagggtaa attgtaccta ttccaaatct ataataaaga
4081 tttttcagct tatagcaaag ggcgaccaaa tctacatact ttatattgga aagcgctgtt
4141 tgatgagaga aatcttcaag atgtggttta taagctaaat ggtgaggcag agctttttta
4201 tcgtaaacaa tcaataccta aaaaaatcac tcacccagct aaagaggcaa tagctaataa
4261 aaacaaagat aatcctaaaa aagagagtgt ttttgaatat gatttaatca aagataaacg
4321 ctttactgaa gataagtttt tctttcactg tcctattaca atcaatttta aatctagtgg
4381 agctaataag tttaatgatg aaatcaattt attgctaaaa gaaaaagcaa atgatgttca
4441 tatattaagt atagatagag gtgaaagaca tttagcttac tatactttgg tagatggtaa
4501 aggcaatatc atcaaacaag atactttcaa catcattggt aatgatagaa tgaaaacaaa
4561 ctaccatgat aagcttgctg caatagagaa agatagggat tcagctagga aagactggaa
4621 aaagataaat aacatcaaag agatgaaaga gggctatcta tctcaggtag ttcatgaaat
4681 agctaagcta gttatagagt ataatgctat tgtggttttt gaggatttaa attttggatt
4741 taaaagaggg cgtttcaagg tagagaagca ggtctatcaa aagttagaaa aaatgctaat
4801 tgagaaacta aactatctag ttttcaaaga taatgagttt gataaaactg ggggagtgct
4861 tagagcttat cagctaacag caccttttga gacttttaaa aagatgggta aacaaacagg
4921 tattatctac tatgtaccag ctggttttac ttcaaaaatt tgtcctgtaa ctggttttgt
4981 aaatcagtta tatcctaagt atgaaagtgt cagcaaatct caagagttct ttagtaagtt
5041 tgacaagatt tgttataacc ttgataaggg ctattttgag tttagttttg attataaaaa
5101 ctttggtgac aaggctgcca aaggcaagtg gactatagct agctttggga gtagattgat
5161 taactttaga aattcagata aaaatcataa ttgggatact cgagaagttt atccaactaa
5221 agagttggag aaattgctaa aagattattc tatcgaatat gggcatggcg aatgtatcaa
5281 agcagctatt tgcggtgaga gcgacaaaaa gttttttgct aagctaacta gtgtcctaaa
5341 tactatctta caaatgcgta actcaaaaac aggtactgag ttagattatc taatttcacc
5401 agtagcagat gtaaatggca atttctttga ttcgcgacag gcgccaaaaa atatgcctca
5461 agatgctgat gccaatggtg cttatcatat tgggctaaaa ggtctgatgc tactaggtag
5521 gatcaaaaat aatcaagagg gcaaaaaact caatttggtt atcaaaaatg aagagtattt
5581 tgagttcgtg cagaatagga ataactaatt cattcaagaa tatattaccc tgtcagttta
5641 gcgactatta cctctttaat aatttgcagg ggaattattt tagtaatagt aatatacaca
5701 agagttattg attatatgga aaattatatt tagataacat ggttaaatga ttttatattc
5761 tgtccttact cgatatattt gcataatatc tatagtaatg cctcagatac tacatactat
5821 tcatctagcc aaacaaaagg gcgcgatgct cataaaagta tcgataaagg aatctatagt
5881 accaaaaaag atgacctgat cggtatcgat gttattaacc ataaatatgg tttggttggt
5941 aaaattgatg tttttcataa agataagggc ttacttgtgg agagaaaaag gcaaatcaag
6001 actatctatg atggctataa atatcagctt tatgcgcaat atttttgtct ccaagagatg
6061 ggctatgatg tcaaagccat taaattttat tcgatggttg ataataaatc atacccaata
6121 gctataccaa cttcagctga gttagaaaag tttgaaaaac atattcaaac aatcaagcaa
6181 tataatccaa tggataactc atttaggcaa aatattgaaa agtgtaaatt ttgtatatat
6241 gcaaacttat gtgataaaac ggacttgtag attatgttta gtaaaaatga tattgaatca
6301 aagaatatag tttttgttaa tatttttgat ggagtgaaac ttagtctatc attggggaat
6361 atagttataa aagataaaga aactgatgag gtgaaaacta agctttctgt tcataaagtt
6421 cttgcattgt ttatcgtagg taatatgacg atgacctcgc aacttttaga gacctgtaag
6481 aaaaatgcta tacagctagt ttttatgaaa aatagcttta gaccatatct atgttttggt
6541 gatattgctg aggctaattt tttagctaga tataagcaat atagtgtagt tgagcaagat
6601 ataagtttag caaggatttt tataacatca aagatacgca atcaacataa cttagtcaaa
6661 agcctaagag ataaaactcc agagcagcaa gagatagtca aaaagaataa acagctaata
6721 gcagagttag aaaatacaac aagcctagcg gagctaatgg gtatagaggg caatgttgcc
6781 aaaaatttct tcaaaggatt ctatggacat ttagatagtt ggcaagggcg caaacctaga
6841 ataaaacagg atccatataa tgttgtttta gacttgggct atagtatgtt gtttaatttt
6901 gtagagtgtt ttttgcgact ttttggcttt gatttataca agggcttttg tcatcagact
6961 tggtataagc gtaaatccct agtttgtgac tttgttgagc catttagatg tatagtggat
7021 aaccaagtta gaaaatcatg gaatctcggg caattttctg tagaggattt tggttgcaaa
7081 aatgagcagt tttatataaa aaaagataaa acaaaagact actcaaaaat actttttgcc
7141 gagattatca gctacaagct agagatattt gaatatgtaa gagaatttta tcgtgccttt
7201 atgcgaggca aagaaattgc agagtatcca atattttgtt atgaaactag gagggtgtat
7261 gttgatagtc agttatgatt ttagtaataa taaagtacgt gcaaagtttg ccaaatttct
7321 agaaagttat ggtgtacgtt tacaatattc ggtatttgag ctcaaatata gcaagagaat
7381 gttagacttg attttagctg agatagaaaa taactatgta ccactattta caaatgctga
7441 tagtgtttta atctttaatg ctccagataa agatgtgata aaatatggtt atgcgattca
7501 tagagaacaa gaggttgttt ttatagacta aaaattgcaa accttagtct ttatgttaaa
7561 ataactacta agttcttaga gatatttaaa aatatgactg ttgttatata tcaaaatgct
7621 aaaaaaatca tagattttag gtcttttttt gctgatttag gcaaaaacgg gtctaagaac
7681 tttaaataat ttctactgtt gtagatgaga agtcatttaa taaggccact gttaaaagtc
7741 taagaacttt aaataatttc tactgttgta gatgctacta ttcctgtgcc ttcagataat
7801 tcagtctaag aactttaaat aatttctact gttgtagatg tctagagcct tttgtattag
7861 tagccggtct aagaacttta aataatttct actgttgtag attagcgatt tatgaaggtc
7921 atttttttgt ctagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg
7981 caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg
8041 ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt
8101 ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc
8161 tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg
8221 cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga
8281 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
8341 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
8401 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
8461 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
8521 gcttcctaat gcaggagtcg cataagggag agcgtcgacc gatgcccttg agagccttca
8581 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
8641 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
8701 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
8761 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
8821 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
8881 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
8941 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
9001 gatcgctcgc ggctcttacc agcctaactt cgatcattgg accgctgatc gtcacggcga
9061 tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat
9121 accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa
9181 tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt
9241 cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc
9301 catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg
9361 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta
9421 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc
9481 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg
9541 gaagtcccct acgtgctgct gaagttgccc gcaacagaga gtggaaccaa ccggtgatac
9601 cacgatacta tgactgagag tcaacgccat gagcggcctc atttcttatt ctgagttaca
9661 acagtccgca ccgctgccgg tagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
9721 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgcccaac
9781 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgcc ctgcaccatt
9841 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
9901 ttaacgaagc gctaaccgtt tttatcaggc tctgggaggc agaataaatg atcatatcgt
9961 caattattac ctccacgggg agagcctgag caaactggcc tcaggcattt gagaagcaca
10021 cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta
10081 tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt
10141 catccgctta ttatcactta ttcaggcgta gcaaccaggc gtttaagggc accaataact
10201 gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
10261 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
10321 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
10381 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
10441 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
10501 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
10561 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
10621 tatcaccagc tcaccgtctt tcattgccat acg
//
